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Selected reaction monitoring assays in mesenchymal stem cells from osteoarthritis patients |
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| Authors: | Emilio Camafeita José-Ramón Lamas Enrique Calvo Pilar Tornero-Esteban Juan-Antonio López Benjamín Fernández-Gutiérrez |
| Institution: | 1.Unidad de Proteómica, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain;2.Rheumatology Service, Hospital Clínico San Carlos, Profesor Martín Lagos s/n, Madrid E-28040, Spain |
| Abstract: | Osteoarthritis (OA) is considered the most prevalent form of arthritis. The aim of this study was to verify potential protein OA biomarkers by applying Selected Reaction Monitoring (SRM) assays to protein extracts obtained from Bone Marrow-Mesenchymal Stem Cells (BM-MSCs) isolated from OA patients.BM aspirates were obtained from the femoral channel of OA patients at the time of surgery and from the femoral channel of hip fracture subjects without OA during hip joint replacement surgery for the treatment of subcapital fracture.SRM results verified the differential expression of several protein biomarkers in BM-MSCs from OA patients. |
| Keywords: | Osteoarthritis Mesenchymal stem cells Selected reaction monitoring |