双歧杆菌及其发酵液的抗肿瘤机制的研究

目的 通过比较双歧杆菌及其发酵液的抗肿瘤作用，对其抑瘤作用和相关机制进行初步探讨。方法 取BALB／c小鼠，分别予H22细胞和S180细胞腹腔荷瘤，荷瘤后给予不同的治疗。分别观察2种肿瘤细胞荷瘤小鼠的生存期。右腋下荷瘤后，进行病理切片观察。MTT法计算给予不同方法后的抑瘤率。并计算淋巴细胞转化率。制备电镜标本。观察其超微结构。结果 双歧杆菌死菌液、活菌液能够延长荷瘤小鼠的生存期，而双歧杆菌发酵液不能延长其生存期，病理切片可见作用后的肿瘤组织内部和间质有大量炎性细胞浸润。双歧杆菌死菌液、活菌液对肿瘤细胞株有抑杀作用。电镜观察死菌液作用后的H22细胞可见典型的凋亡表现。结论 双歧杆菌死菌液、活菌液在体内外均有较好的抑瘤作用，其发酵液并未显示出抑瘤作用。说明双歧杆菌的抗肿瘤作用主要来源于菌体的作用。

Experimental study on anti-tumor effect with Bifidobacterium and its broth

Objective By comparing Bifidobacterium and its broth's anti-tumor effect,we initially understand its anti-tumor mechanism.Methods BALB/c mice bearing H22 and S180 were given to different methods,The therapeutic effect was determined by life span.Mice bearing H22 substantial tumor under right axilla,The therapeutic effect was determined by means of pathological examination of tumor tissue.MTT assay calculats the Inhibition rate,and calculats lymphocyte transformation rate.Electron microscope was used to study the cell morphology.Results Thanato-Bifidobacterium and vivi-Bifidobacterium could prolong the life span of mice bearing tumor,inhibited growth of tumor,but its broth could't prolong the life span.Thanato-Bifidobacterium and vivi-Bifidobacterium could inhibit and kill tumor cells.In pathological section,we may observe the growth of tumor tissues affected by Bifidobacterium were inhibited,and may observe a lot of phlegmonosis cells in tumor tissue and its interstitial substance.Conclusion Thanato-Bifidobacterium and vivi-Bifidobacterium have signifycant anti-tumor effect in vitro and in vivo,could prolong the life span of mice bearing tumors and inhibit substantial tumor cells,but its broth did not show anti-tumor effect,demonstrate that the anti-tumor effect of Bifidobacterium chiefly origins from its thalline.