Molecule-level imaging of Pax6 mRNA distribution in mouse embryonic neocortex by molecular interaction force microscopy |
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| Authors: | Yu Jin Jung Yu Shin Park Ki-Jun Yoon Young-Yun Kong Joon Won Park and Hong Gil Nam |
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| Institution: | 1.Center for Integrated Molecular Systems, Department of Chemistry, 2.National Core Research Center for Systems Bio-Dynamics, 3.Division of Molecular and Life Sciences and 4.School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, San 31 Hyoja-dong, Pohang, 790-784, South Korea |
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| Abstract: | Detection of the cellular and tissue distributions of RNA species is critical in our understanding of the regulatory mechanisms underlying cellular and tissue differentiation. Here, we show that an atomic force microscope tip modified with 27-acid dendron, a cone shaped molecule with 27 monomeric units forming its base, can be successfully used to map the spatial distribution of mouse Pax6 mRNA on sectioned tissues of the mouse embryonic neocortex. Scanning of the sectioned tissue with a 30-mer DNA probe attached to the apex of the dendron resulted in detection of the target mRNA on the tissue section, permitting mapping of the mRNA distribution at nanometer resolution. The unprecedented sensitivity and resolution of this process should be applicable to identification of molecular level distribution of various RNAs in a cell. |
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