Prostaglandin production by methylcholanthrene-transformed mouse BALB/3T3: Effect of oxidative phosphorylation inhibitors |
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Authors: | Sheng-Shung Pong Lawrence Levine |
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Institution: | Department of Biochemistry, Brandeis University Waltham, Massachusetts 02154 USA |
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Abstract: | Oligomycin, antimycin, and 2,4-dinitrophenol, compounds that are known to inhibit oxidative phosphorylation by different mechanisms, inhibit the production of prostaglandins by serum-stimulated MC5-5 cells. The prostaglandin production that is stimulated by thrombin and bradykinin is inhibited by 2,4-dinitrophenol. Prostaglandin synthesis by MC5-5 cells from exogenously-supplied arachidonic acid, however, is not affected by 2,4-dinitrophenol. Antimycin and 2,4-dinitrophenol also inhibit the serum-stimulated release of arachidonic acid from the cellular lipids, suggesting that it is the expression of phospholipase activity, a prerequisite for synthesis of prostaglandins by MC5-5 cells, that is dependent on oxidative phosphorylation. |
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