Role of iron chelators in growth-promoting effect on mouse hybridoma cells in a chemically defined medium |
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Authors: | Noritsugu Yabe Miwa Kato Yutaka Matsuya Isao Yamane Muneaki Iizuka Hiroyuki Takayoshi Kiyokazu Suzuki |
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Institution: | (1) Division of cell Biology, The Research Institute for Tuberculosis and Cancer, Tohoku University, 4-1 Seiryomachi, 980 Sendai, Miyagi, Japan;(2) Laboratory of Biochemistry, Nippon Zenvaku Kogyo Co., Ltd., 1-1 Tairanoue, Asakamachi, Koriyama, 969-05 Fukushima, Japan;(3) Laboratory of Biotechnology, Kohjin Co., Ltd., 3-4-5 Asabano, Sakado, 350 Saitama, Japan |
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Abstract: | Summary The role of various iron chelators on the multiplication of mouse hybridoma cells in an albumin-free, transferrin-deficient
defined medium was investigated. Fe(III)-dihydroxyethylglycine, Fe(III)-glycylglycine, Fe(III)-ethylenediamine-N,N′-dipropionic acid, or Fe(III)-iminodiacetic acid supported the excellent growth of the cells. In addition, the growth of the
iron-starved cells, which had been preincubated in a protein-, iron- and chelator-free defined medium, restored rapidly when
the medium was supplemented with holotransfeerrin, ferric iron, and chelator compared to that when supplemented with holotransferin,
but without iron and chelator. The results suggest that such chelators modulate a progression of transferrn cycle in the presence
of transferin and ferric iron. An alternative explantation is that there is a decrease in generation of iron-catalyzed free
radicals. |
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Keywords: | iron chelator transferrin-independent culture transferrin cycle iron-catalyzed free radicals hydridoma cells |
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