Bom m 9 from Bombyx mori is a novel protein related to asthma |
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| Authors: | Jianhong Zuo Mingsheng Lei Rui Yang Zhigang Liu |
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| Affiliation: | 1. State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, Guangdong;2. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060;3. Medical School, University of South China, Hunan;4. Department of Respiratory and Critical Care Medicine, Zhangjiajie City Hospital, Zhangjiajie, China;5. Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, China |
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| Abstract: | The silkworm (Bombyx mori) can cause severe IgE‐mediated allergic disease, however, the mechanism remains unclear. The aim of this study was to investigate the immunologic mechanism by which silkworms induce allergy. Whole silkworm pupa proteins were separated by SDS‐PAGE and 2‐D PAGE. Then, IgE‐binding proteins were detected by immunoblotting with sera of patients having an allergy to Bombyx mori. After tryptic digestion, the peptides of IgE‐binding proteins were analyzed by matrix‐assisted laser desorption ionization tandem time‐of‐flight mass spectrometry or tandem mass spectrometry. Database searches were used to identify allergens in silkworm pupa, after which Bom m 9 was to construct an asthma model. Thus, in the current study, a mouse asthma model was constructed with Bom m 9. |
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| Keywords: | asthma Bom m 9 proteomics silkworm (Bombys mori) pupa |
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