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Replication of IMR‐32‐adapted JC virus clones in human embryonic kidney cells |
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| Authors: | Souichi Nukuzuma Shigeki Sugiura Kazuo Nakamichi Masanori Kameoka Chiyoko Nukuzuma Takafumi Tasaki Tsutomu Takegami |
| Institution: | 1. Department of Infectious Diseases, Kobe Institute of Health, Kobe;2. Medical Genetics Research Center, Nara Medical School, Nara;3. Department of Virology 1, National Institute of Infectious Diseases, Tokyo;4. Department of International Health, Kobe University Graduate School of Health Sciences, Kobe;5. Tokyo SOARA Clinic, Shinagawa‐ku, Tokyo;6. Divison of Protein Regulation Research, Medical Research Institute, Kanazawa Medical University, Ishikawa;7. Division of Molecular Oncology and Virology, Medical Research Institute, Kanazawa Medical University, Ishikawa, Japan |
| Abstract: | It has been difficult to study JCV replication because of its restricted host range. In this study, JCV replication was examined using different clones in 293 cells. RT‐PCR assay revealed that large T antigen expression in cells transfected with IMR‐32‐adapted JCVs was significantly greater than in those transfected with Mad‐1 or CY. DNA replication assay and viral load verified that the IMR‐32‐adapted JCVs were replication‐competent in 293 cells, but not Mad‐1 or CY JCVs. These results suggest that a 293 culture system with IMR‐32‐adapted JCVs may be a useful tool for assessing replication of JCV in vitro. |
| Keywords: | 293 cells DNA replication assay IMR‐32‐adapted JCVs large T antigen |