Simultaneous biodegradation of creosote-polycyclic aromatic hydrocarbons by a pyrene-degrading <Emphasis Type="Italic">Mycobacterium</Emphasis> |
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Authors: | Zaira López Joaquim Vila José-Julio Ortega-Calvo Magdalena Grifoll |
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Institution: | (1) Departament de Microbiologia, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain;(2) Instituto de Recursos Naturales y Agrobiología, C.S.I.C., Apartado 1052, 41080 Seville, Spain;(3) Present address: Departamento Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega, Universidad de Guadalajara, Ocotlán, Jalisco, México |
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Abstract: | When incubated with a creosote-polycyclic aromatic hydrocarbons (PAHs) mixture, the pyrene-degrading strain Mycobacterium sp. AP1 acted on three- and four-ring components, causing the simultaneous depletion of 25% of the total PAHs in 30 days.
The kinetics of disappearance of individual PAHs was consistent with differences in aqueous solubility. During the incubation,
a number of acid metabolites indicative of distinctive reactions carried out by high-molecular-weight PAH-degrading mycobacteria
accumulated in the medium. Most of these metabolites were dicarboxylic aromatic acids formed as a result of the utilization
of growth substrates (phenanthrene, pyrene, or fluoranthene) by multibranched pathways including meta- and ortho-ring-cleavage reactions: phthalic acid, naphthalene-1,8-dicarboxylic acid, phenanthrene-4,5-dicarboxylic acid, diphenic acid,
Z-9-carboxymethylenefluorene-1-carboxylic acid, and 6,6′-dihydroxy-2,2′-biphenyl dicarboxylic acid. Others were dead-end products
resulting from cometabolic oxidations on nongrowth substrates (fluorene meta-cleavage product). These results contribute to the general knowledge of the biochemical processes that determine the fate
of the individual components of PAH mixtures in polluted soils. The identification of the partially oxidized compounds will
facilitate to develop analytical methods to determine their potential formation and accumulation in contaminated sites.
An erratum to this article can be found at |
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Keywords: | Biodegradation PAHs Mycobacterium Metabolites |
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