Direct interaction of Cucurbitacin E isolated from Alsomitra macrocarpa to actin filament |
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| Authors: | Keiko Momma Yuko Masuzawa Naomi Nakai Moeko Chujo Akira Murakami Noriyuki Kioka Yasunori Kiyama Toru Akita Masaya Nagao |
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| Affiliation: | (1) Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan;(2) Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan;(3) Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan;(4) Nippon Shinyaku Co., Ltd, Kyoto, Japan |
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| Abstract: | A methanol extract of Alsomitra macrocarpa leaves and branches induced a marked alteration of cell morphology in a human stellate cell line (LX-2). Similar morphologic alterations were observed in several other cell lines. Active compound was purified from the extract and determined to be cucurbitacin E (Cuc E). It has been known that Cuc E causes marked disruption of the actin cytoskeleton, supporting our observation, but how Cuc E altered the actin cytoskeleton has not been elucidated. By using the standard fluorescence assay using copolymerization and depolymerization of native and pyrene labelled actin, this study revealed that Cuc E interacted directly with actin consequently stabilizing the polymerized actin. When NIH-3T3 cells exogenously expressing YFP-labeled actin were treated with Cuc E, firstly the aggregation of globular actin and secondly the aggregation of actin including disrupted fibrous actin in the cells was observed. |
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| Keywords: | Alsomitra macrocarpa Cucurbitacin E Cell morphology Actin Cytoskeleton |
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