Biological and methodological implications of prostaglandin involvement in mouse brain lipid peroxidation measurements |
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Authors: | Ranjan Bose Garnette R. Sutherland Carl Pinsky |
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Affiliation: | (1) Department of Pharmacology and Therapeutics, University of Manitoba, Faculty of Medicine, 770 Bannatyne Avenue, R3E 0W3 Winnipeg, Manitoba, Canada;(2) Department of Surgery (Neurosurgery), University of Manitoba, Faculty of Medicine, 770 Bannatyne Avenue, R3E 0W3 Winnipeg, Manitoba, Canada |
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Abstract: | Enhanced cyclooxygenase-mediated prostaglandin (PG) turnover occurring during sacrifice and biochemical processing of tissues also generates malondialdehyde (MDA), a product of lipid peroxidation (LPO). Studies reporting on LPO estimated by thiobarbituric acid reactive substances (TBARS) have failed to consider such artefactual increases. This study reports the relative proportion of PG metabolism-derived MDA (PG-MDA) in mouse brain regions during the TBARS assay. The cyclooxygenase inhibitor indomethacin significantly lowered MDA in fronto-parietal cortex and corpus striatum. Indomethacin (50–800 g/ml, in vitro) increased estimated TBARS in whole brain. Such enhancement was absent when indomethacin (20–80 g/sample) was added to the MDA standard curve, reflecting its interaction with TBARS other than MDA. PG-MDA contributes as much as 15% to the total estimated value of MDA in fronto-parietal cortex and corpus striatum and must be corrected for in LPO studies. |
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Keywords: | Lipid peroxidation malondialdehyde prostaglandin indomethacin thiobarbituric acid reactive substances |
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