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Characterization of monoclonal lipid A antibodies with synthetic lipid A analogues |
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| Authors: | S. Arata J. Mashimo N. Kasai K. Okuda Y. Aihara S. Kotani H. Takada T. Shiba S. Kusumoto T. Shimamoto N. Kusunose |
| Affiliation: | Department of Microbial Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan;Department of Bacteriology, Yokohama City University School of Medicine, Yokohama, Japan;Osaka College of Medical Technology, Osaka, Japan;Department of Microbiology and Oral Microbiology, Osaka University Dental School, Osaka, Japan;Department of Chemistry, Faculty of Science, Osaka University, Osaka, Japan |
| Abstract: | Abstract The specificity of monoclonal antibodies directed against the Salmonella minnesota R595 lipid A and the structural requirements of lipid A epitopes were studied with chemically synthesized lipid A analogues by enzyme-linked immunosorbent assay and its inhibition test. The results suggest that lipid A has specific and common epitopes, in which the specificities are derived from the chemical and conformational structures of the backbone and/or acyl groups. |
| Keywords: | Monoclonal antibody Lipid A epitope Synthetic lipid A analogue |