Reconstruction of non-uniformly sampled five-dimensional NMR spectra by signal separation algorithm |
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| Authors: | Krzysztof Kosiński Jan Stanek Michał J Górka Szymon Żerko Wiktor Koźmiński |
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| Institution: | 1.Faculty of Chemistry, Biological and Chemical Research Centre,University of Warsaw,Warsaw,Poland;2.Faculty of Physics, Division of Biophysics, Institute of Experimental Physics,University of Warsaw,Warsaw,Poland |
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| Abstract: | A method for five-dimensional spectral reconstruction of non-uniformly sampled NMR data sets is proposed. It is derived from the previously published signal separation algorithm, with major alterations to avoid unfeasible processing of an entire five-dimensional spectrum. The proposed method allows credible reconstruction of spectra from as little as a few hundred data points and enables sensitive resonance detection in experiments with a high dynamic range of peak intensities. The efficiency of the method is demonstrated on two high-resolution spectra for rapid sequential assignment of intrinsically disordered proteins, namely 5D HN(CA)CONH and 5D (HACA)CON(CO)CONH. |
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