H+ translocation by weak acid uncouplers is independent of H+ electrochemical gradient |
| |
Authors: | Jaromír Plášek David Babuka Milan Hoefer |
| |
Affiliation: | 1.Faculty of Mathematics and Physics,Charles University,Prague,Czech Republic;2.Institute of Cellular and Molecular Botany,University of Bonn,Bonn,Germany |
| |
Abstract: | According to the common view, weak acid uncouplers increase proton conductance of biological (and phospholipid bilayer) membranes, thus effecting H+ fluxes driven by their electrochemical gradients. Under certain conditions, however, uncouplers can induce unexpected effects opposite to the dissipation of H+ gradients. Results are presented here demonstrating CCCP-induced proton influx into Saccharomyces cerevisiae cytosol driven by the electrochemical potentials of CCCP and its CCCP? anions, independent of electrochemical H+-gradient. Another view of week acid uncouplers’ action is proposed that is logically consistent with these observations. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|