Pathogenic potential of six isolates of entomopathogenic nematodes (Rhabditida: Steinernematidae) from Vietnam |
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| Authors: | KL?Phan L?Tirry Email author" target="_blank">M?MoensEmail author |
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| Institution: | (1) Institute of Ecology and Biological Resources, National Centre for Science and Technology, Hoang Quoc Viet 18, Nghiado, Caugiay, Hanoi, Vietnam;(2) Laboratory for Agrozoology, Department for Crop Protection, Gent University, Coupure 653, B-9000 Gent, Belgium;(3) Ctr voor Landbouwkundig Onderzoek, Gewasbescherming, Burg. van Gansberghelaan 96, B-9820 Merelbeke, Belgium |
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| Abstract: | The potential of six Steinernema isolates, isolated from different provinces in Vietnam, was evaluated in the laboratory against Galleria mellonella and Spodoptera littoralis. Steinernema sangi and S. robustispiculum TN24 had the highest penetration rate in both hosts according to a penetration rate assay. The virulence assay showed that S. sangi had a high virulence to both hosts and along with isolate TN38 it was the most mobile among the isolates tested. The migration of S. sangi in sand columns with an insect host at the bottom was significantly higher than in sand columns without insect host. This Steinernema species was the only one that penetrated a host in 24h after migrating 10cm in sand columns at 25°C. Moreover, a multiplication assay showed that S. sangi produced a high number of infective juveniles in G. mellonella. However, all Steinernema isolates tested had low multiplication rates in S. littoralis. |
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| Keywords: | Galleria mellonella migration reproduction Spodoptera littoralis Steinernema robustispiculum S sangi virulence |
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