Localization of the adrenodoxin-binding fragment of cholesterol hydroxylating cytochrome P-450 from adrenal cortex mitochondria

The interaction between cytochrome P-450scc and adrenodoxin has been studied using cleavable cross-linking reagents and limited trypsinolysis. The data obtained indicate that the site responsible for adrenodoxin binding is located on the NH2-terminal fragment F1 of cytochrome P-450scc.