Overexpression of the nucleolar protein nucleophosmin/B23 in collagen lattice-cultured fibroblasts: Potential role in the control of protein synthesis |
| |
Authors: | Lefèvre Fabrice Garnotel Roselyne Georges Nadine Gillery Philippe |
| |
Affiliation: | (1) Laboratoire de Biochimie et Biologie Moléculaire, Faculté de Médecine, 51 rue Cognacq-Jay, 51095 Reims cedex, France |
| |
Abstract: | Fibroblasts cultivated in tridimensional collagen lattices exhibit a downregulation of protein synthesis, related to decreased ribosomal RNA (rRNA) content and half life, when compared to monolayer cultivated cells. The involvement in this process of nucleophosmin/B23, a nucleolar phosphoprotein with ribonuclease properties, was checked. We compared production of nucleophosmin/B23 in monolayer and collagen lattice cultured fibroblasts. A significant increase of nucleophosmin/B23 mRNA levels was noticed in lattice-cultured fibroblasts vs monolayers (+154%, p < 0.05). A concomitant enhancement of nucleolar nucleophosmin/B23 content was found (+112%, p < 0.001). Simultaneously, ribonuclease activity contained in nucleolar extracts from collagen lattice-cultured fibroblasts was significantly increased (+54%, p < 0.01). These data demonstrate that extracellular collagen matrix induces the overexpression of nucleophosmin/B23, and suggest that the regulation of protein syntheses in collagen lattice cultures may be explained, at least partly, by an increased degradation of neosynthesized rRNAs dependent on nucleophosmin. |
| |
Keywords: | fibroblasts collagen lattice cultures nucleophosmin/B23 protein synthesis ribonuclease extracellular matrix |
本文献已被 PubMed SpringerLink 等数据库收录! |
|