Development of a shuttle vector and a conjugative transfer system for Actinobacillus pleuropneumoniae |
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| Authors: | G Lalonde P D O'Hanley |
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| Institution: | Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305. |
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| Abstract: | An original genetic system for Actinobacillus pleuropneumoniae has been developed. A shuttle cloning vector, pYG53, was constructed from the wild-type plasmid pYG10. It permits, in conjunction with electroporation, the introduction of cloned genes into this species. A conjugal transfer system between Escherichia coli and A. pleuropneumoniae involving pYG54, a mobilizable derivative of pYG53, is also described. Conjugation efficiencies of 8.3 x 10(-3) exconjugants per donor can be obtained. |
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