Redox modulation of A-type K+ currents in pain-sensing dorsal root ganglion neurons |
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Authors: | Hsieh Chi-Pan |
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Institution: | Department of Physiology, College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, 1st Section, Taipei 100, Taiwan |
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Abstract: | Redox modulation of fast inactivation has been described in certain cloned A-type voltage-gated K+ (Kv) channels in expressing systems, but the effects remain to be demonstrated in native neurons. In this study, we examined the effects of cysteine-specific redox agents on the A-type K+ currents in acutely dissociated small diameter dorsal root ganglion (DRG) neurons from rats. The fast inactivation of most A-type currents was markedly removed or slowed by the oxidizing agents 2,2′-dithio-bis(5-nitropyridine) (DTBNP) and chloramine-T. Dithiothreitol, a reducing agent for the disulfide bond, restored the inactivation. These results demonstrated that native A-type K+ channels, probably Kv1.4, could switch the roles between inactivating and non-inactivating K+ channels via redox regulation in pain-sensing DRG neurons. The A-type channels may play a role in adjusting pain sensitivity in response to peripheral redox conditions. |
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Keywords: | Dorsal root ganglion A-type K+ channels Redox modulation |
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