Differential expression of individual genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in Lemna gibba |
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Authors: | Jane Silverthorne Charles F Wimpee Takashi Yamada Stephen A Rolfe Elaine M Tobin |
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Institution: | (1) Biology Department and Molecular Biology Institute, University of California, 90024 Los Angeles, CA, USA;(2) Present address: Department of Biology, University of California, 95064 Santa Cruz, CA, USA;(3) Present address: Department of Biology, University of Wisconsin, 53201 Milwaukee, WI, USA;(4) Present address: Department of Molecular Biology, Mitsubishi-Kasei Institute of Life Sciences, 11, Minamiooya, Machida-shi, 194 Tokyo, Japan;(5) Biology Dept., U.C.L.A., 90024 Los Angeles, CA, USA |
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Abstract: | The gene family encoding the small subunit (SSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase in the monocot Lemna gibba contains approximately twelve members. We have isolated six of these genes from a genomic library, and sequenced five of the coding regions. The transit peptide nucleotide sequences are conserved, but less highly than the mature polypeptide coding sequence. The mature polypeptide amino acid sequences are identical to each other and to the sequence deduced from a cDNA clone derived from a seventh gene. Each of the five fully characterized genomic sequences contains a single intron in precisely the same position as the second intron of several dicots. The intron sequences differ in length and are less conserved than the coding sequences.The 3 -untranslated regions of the different genes have been sequenced and used to prepare gene-specific probes. These probes have been used to study the expression levels of individual rbcS sequences. Expression of six of the seven genes can be detected in total RNA isolated from plants grown in continuous light. The levels of RNA encoded by each expressed gene are regulated by the action of phytochrome, but there is variability in the amount of expression of each RNA. |
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Keywords: | gene-specific probes Lemna gibba phytochrome rbcS genes ribulose-1 5-bisphosphate carboxylase/oxygenase small subunit |
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