Analysis of C14-labeled proteins by disc electrophoresis

Ornstein and Davis (1964) have introduced a method, called “disc electrophoresis”, which yields extremely high resolution of proteins electrophoresed in cylindrical columns of polyacrylamide gel. By means of disc electrophoresis, a large number of protein components in a complex mixture can be separated and detected in a single operation. Bands of C¹⁴-labeled proteins in disc electropherograms can be detected using techniques described by Heideman (1964); and Jovin, Chrambach, and Naughton (1964). This report presents an alternative procedure involving autoradiography of dried longitudinal gel slices. The method is relatively uncomplicated and can be used to develop the entire pattern of radioactivity in a gel without significant sacrifice of resolution.