The assay of uridine diphosphoglucose dehydrogenase activity: discrimination from xanthine dehydrogenase activity |
| |
| Authors: | S Mehdizadeh L Bitensky J Chayen |
| |
| Institution: | Unit of Cellular Pharmacology and Toxicology, Robens Institute of Health and Safety, University of Surrey, Guildford, U.K. |
| |
| Abstract: | The biochemical and quantitative cytochemical assays of the activity of uridine diphosphoglucose dehydrogenase (UDPG-D) have produced perplexing results. It is now shown that the perplexity may be due to the possibility that the coenzyme (NAD) required for UDPG-D activity, may be acting as a substrate for a second dehydrogenase, namely xanthine dehydrogenase, which may utilize NAD as its substrate. The activity of UDPG-D can be distinguished selectively by the pH of its optimal activity and by decreasing the concentration of the coenzyme used in the assay. |
| |
| Keywords: | Uridine diphosphoglucose dehydrogenase xanthine dehydrogenase quantitative cytochemistry microdensitometry |
|
|
