Improved methods for the enzymic preparation and chromatography of octulose phsophates |
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| Affiliation: | 1. Department of Chemistry, Presidency University, Bengaluru 560 089, India;2. Department of Chemistry, Donbosco Institute of Technology, Bengaluru 560 074, India;3. Department of Post graduate studies in Chemistry, Bangalore University, Bengaluru 560 001, India |
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| Abstract: | High-yield methods are presented for the synthesis of d-glycero-d-altro-octulose 8-phosphate and 1,8-diphosphate, and d-glycero-ido-octulose 1,8-diphosphate and 8-phosphate. Formate ion-exchange and DEAE-Sephadex A-25 borate column chromatography were used for the isolation of these compounds. A new phenylboronate-containing solvent for p.c. and t.l.c. of sugar phosphates is described. The 13C-n.m.r. assignments of the major contributing structure of d-glycero-d-altro-octulose 8-phosphate in aqueous solution (pH 7.0) are presented. |
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