The cranial sensory ganglia in culture: Differences in the response of placode-derived and neural crest-derived neurons to nerve growth factor |
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| Institution: | 1. Department of Anatomy, St. George''s Hospital Medical School, Cranmer Terrace, Tooting, London SW17 0RE, England;2. Laboratory of Neurobiology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, England;1. Department of Biological Sciences, North Carolina State University, Raleigh, NC, USA;2. W.M. Keck Center for Behavioral Biology, Center for Human Health and the Environment, and Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA;1. Pain Research Center and Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University, 74 Zhongshan Rd. 2, Guangzhou 510080, PR China;2. Department of Anesthesiology, Cancer Center, Sun Yat-Sen University, State Key Laboratory of Oncology in South China, Collaborative, Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou 510060, PR China;3. Department of Pathology, The Red Cross Hospital of Yulin, 1 Jinwang Rd, Yulin 537000, PR China;1. Department of Neurology, Laboratory of Neurological Diseases, Changzhou No. 2 People’s Hospital, The Affiliated Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, Jiangsu 213003, China;2. Department of Neurology, Foshan First People’s Hospital, The Affiliated Foshan Hospital of Zhongshan University, 81 North Lingnan Avenue, Chancheng District, Foshan, Guangdong 52800, China;3. Department of Physiology and Neuroscience Program, Michigan State University, East Lansing, MI 48824, United States |
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| Abstract: | Explants of cranial sensory ganglia and dorsal root ganglia from embryonic chicks of 4 to 16 days incubation (E4 to E16) were grown for 24 hr in collagen gels with and without nerve growth factor (NGF) in the culture medium. NGF elicited marked neurite outgrowth from neural crest-derived explants, i.e., dorsal root ganglia, the dorsomedial part of the trigeminal ganglion, and the jugular ganglion. This response was first observed in ganglia taken from E6 embryos, reached a maximum between E8 and E11, and gradually declined through E16. Explants in which the neurons were of placodal origin varied in their response to NGF. There was negligible neurite outgrowth from explants of the ventrolateral part of the trigeminal ganglion and the vestibular ganglion grown in the presence of NGF. The geniculate, petrosal, and nodose ganglia exhibited an early moderate response to NGF. This was first evident in ganglia taken from E5 embryos, reached a maximum by E6, and declined through later ages, becoming negligible by E13. Dissociated neuron-enriched cultures of vestibular, petrosal, jugular, and dorsal root ganglia were established from embryos taken at E6 and E9. At both ages NGF elicited neurite outgrowth from a substantial proportion of neural crest-derived neurons (jugular and dorsal root ganglia) but did not promote the growth of placode-derived neurons (vestibular and petrosal ganglia). Our findings demonstrate a marked difference in the response of neural crest and placode-derived sensory neurones to NGF. The data from dissociated neuron-enriched cultures suggest that NGF promotes survival and growth of sensory ganglionic neurons of neural crest origin but not of placodal origin. The data from explant cultures suggest that NGF promotes neurite outgrowth from placodal neurons of the geniculate, petrosal, and nodose ganglia early in their ontogeny. However, we argue that this fibre outgrowth emanates not from the placodal neurons but from neural crest-derived cells which normally give rise only to satellite cells of these ganglia. |
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