A multiple-column approach to the methylation analysis of plant cell-walls |
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| Affiliation: | 1. Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Centre, Stockholm, SE10691, Sweden;2. School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wuxing Street, Taipei 11031, Taiwan;3. Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta T1J 4B1, Canada;4. Institute of Chemistry, Academia Sinica, No. 128 Academia Road, Section 2, Nankang District, Taipei, Taiwan;5. School of Biological Sciences, The University of Auckland, Auckland Mail Centre, Private Bag 92019, Auckland 1142, New Zealand;1. Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Ocean University of China, Qingdao 266003, China;2. Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;1. Institute of Chemistry of Renewable Resources, Department of Chemistry, University of Natural Resources and Life Sciences Vienna, Konrad-Lorenz-Straße 24, 3430, Tulln an der Donau, Austria;2. Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, Mulyorejo, Surabaya, 60115, Indonesia |
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| Abstract: | A procedure has been developed for the improved analysis of the neutral-sugar glycosidic linkages in plant cell-walls, utilising capillary g.l.c. and columns of three different phases for the separation of the products of methylation analysis. Retention coefficients are reported for a wide range of partially methylated alditol acetates on columns of CP-Sil88 and a bonded phase (BP-1) equivalent to OV-1. Using these phases and SP-1000, all cases of co-chromatography can be resolved. Computers were used to process the large amounts of data produced, to identify peaks and to assist in merging the results obtained using the three phases. |
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