Anaerobic metabolism of primary and secondary forms of Photorhabdus luminescens |
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Authors: | Bettina M. Rosner Jerald C. Ensign Bernhard Schink |
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Affiliation: | Department of Microbiology, Bose Institute, P-1 / 12, CIT Scheme VIIM, Calcutta 700054, India |
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Abstract: | Abstract A Vibrio cholerae O1 strain (1150) of the EITor biotype and Ogawa serotype with haemagglutination (HA) activity was subjected to TnphoA mutagenesis. Out of several mutants isolated, one HA− and another HA+ mutant were further characterised. The HA− mutant showed about 50% reduction in its intestinal adherence capacity in vitro and about 9-fold decrease of its colonisation ability in vivo, as compared to the wild-type strain. Subsequent studies showed that the HA activity of strain 1150 was mediated by a mannose-sensitive haemagglutinin (MSHA). Thus, the phenotypic expression of MSHA appears to be partly responsible for the intestinal adherence and colonisation properties of strain 1150. |
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Keywords: | Vibrio cholerae Transposon mutagenesis Mannose-sensitive hemagglutinin Intestinal adherence Colonization |
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