赤霉素GA3调节黄芩组织培养中芽和根的形成

应用组织培养技术对黄芩进行外源激素调控研究。在培养不同时间进行的不同培养基之间的转移培养研究表明,组织培养条件下,在培养基中添加赤霉素,可显著刺激黄芩外植体芽的形成,同时抑制根的生长。在加有GA3的IAA培养基上,GA3显著影响黄芩组织培养物中的黄酮含量。在黑暗条件下,开始在2.5μmol/LIAA培养基中培养6d,随后转移到5μmol/LGA3培养基上培养,黄芩外植体中黄岑苷、黄岑素和汉黄芩苷的含量最高,分别为14.90,2.70和0.54μgmg-1(干重)。

Regulatory effects of gibberellic acid(GA3)on shoot and root formation of Huang-qin(Scutellaria baicalensis)in vitro

Influence of exogenously applied gibberellic acid(GA3)on shoot and root formation and development in shoot tip and stem tissue cultures of Huang-qin(Scutellaria baicalensis), an important medicinal plant, was investiga- ted. GA3 application at the concentrations of 1-20 μmol/L resulted in the significant enhancement of shoot formation and growth while it reduced root formation and development. In different transferring tests,the exogenously applied growth regulators GA3 and IAA resulted in significant changes in the concentrations of major flavonoids in Huang-qin tissues. The highest concentrations of baicalin, baicalein and wogonin were 14. 90,2. 70 and 0.54 μg · mg-1 (dry weight), respectively, when the explants were pre-cultured on 2.5 μmol/L IAA medium for 6 days before transferred to MSO medium containing GA3 at 5 μmol/L in continuous darkness.