Use of the two-hybrid system to identify Rab-interacting proteins |
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| Authors: | Janoueix-Lerosey I Goud B |
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| Institution: | INSERM U 509, Laboratoire Mécanismes moléculaires du transport intracellulaire, UMR CNRS 144, Institut Curie, 26 rue d'Ulm, Paris Cedex 05, 75248, France. |
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| Abstract: | The yeast two-hybrid system has been useful for identifying many partners and effectors of small GTPases of the Rab family. We describe here such a screen using Rab6, a protein involved in the regulation of intracellular transport at the level of the Golgi apparatus, as bait. |
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