Kinetics of chlorotetracycline uptake in Staphylococcus aureus by a fluorescence technique |
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Authors: | M E Dockter J A Magnuson |
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Affiliation: | Graduate Program in Biochemistry and Biophysics, Department of Chemistry, Washington State University, Pullman, Washington 99163 USA |
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Abstract: | The antibiotic chlorotetracycline (CTC) is used as a fluorescent chelate probe to investigate the kinetics of its uptake into . CTC binds to divalent cations in an aqueous solution with enhanced fluorescence. This fluorescence is polarity dependent, being higher in apolar solutions. Upon addition of CTC to dispersions of , a time dependent fluorescence enhancement is detected demonstrating that the CTC-divalent cation complex migrates into the apolar regions of the membrane. This uptake, which follows saturation kinetics, is energy dependent. A Km of 162 μ was obtained for CTC concentration ranges of 0.2–100 μgm/ml. |
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