Extracellular overexpression of recombinant <Emphasis Type="Italic">Thermobifida fusca</Emphasis> cutinase by alpha-hemolysin secretion system in <Emphasis Type="Italic">E. coli</Emphasis> BL21(DE3) |
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| Authors: | Lingqia Su Sheng Chen Li Yi Ronald W Woodard Jian Chen Jing Wu |
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| Institution: | (1) State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Ave, Wuxi, Jiangsu, 214122, China;(2) School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Ave, Wuxi, Jiangsu, 214122, China;(3) Department of Medicinal Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA |
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| Abstract: | Background Extracellular expression of proteins has an absolute advantage in a large-scale industrial production. In our previous study,
Thermobifida fusca cutinase, an enzyme mainly utilized in textile industry, was expressed via type II secretory system in Escherichia coli BL21(DE3), and it was found that parts of the expressed protein was accumulated in the periplasmic space. Due to the fact
that alpha-hemolysin secretion system can export target proteins directly from cytoplasm across both cell membrane of E. coli to the culture medium, thus in the present study we investigated the expression of cutinase using this alpha-hemolysin secretion
system. |
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