Production of ginsenoside and polysaccharide by two-stage cultivation of Panax quinquefolium L. cells |
| |
| Authors: | Juan Wang Wen-Yuan Gao Jian Zhang Bei-Mei Zuo Li-Ming Zhang Lu-Qi Huang |
| |
| Affiliation: | (1) School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, 300072 Tianjin, People’s Republic of China;(2) School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 300193 Tianjin, People’s Republic of China;(3) Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, 300457 Tianjin, People’s Republic of China;(4) Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, People’s Republic of China; |
| |
| Abstract: | Based on the results of carbon source consumption in cell suspension culture of Panax quinquefolium L., 30 g L−1 sucrose was fed into a 5-L stirred tank bioreactor on day 16 of culture to enhance cell density and metabolite production. Using a fed-batch cultivation strategy, polysaccharide production was enhanced to 1.608 g L−1, which was 1.96-fold greater than with batch cultivation. The maximum saponin yield (7.828 mg L−1) was obtained on day 24 and was about 36% higher than the yields obtained using batch cultivation. In a two-stage culture process, a combined treatment with sucrose, lactoalbumin hydrolysate, and methyl jasmonate caused a significant increase in total saponin yield (31.52 mg L−1) in cell cultures after 27 d. This value represents an increase of 4.03-fold compared with the total saponin yield in fed-batch cultivation. The two-stage culture mode provided the best method for the in vitro production of secondary metabolites from P. quinquefolium. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
