Advanced back-cross QTL analysis of tomato. II. Evaluation of near-isogenic lines carrying single-donor introgressions for desirable wild QTL-alleles derived from Lycopersicon hirsutum and L. pimpinellifolium |
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| Authors: | D. Bernacchi T. Beck-Bunn D. Emmatty Y. Eshed S. Inai J. Lopez V. Petiard H. Sayama J. Uhlig D. Zamir S. Tanksley |
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| Affiliation: | (1) Department of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, Ithaca, NY 14853-1902, USA Fax: +1 607 255 6683 E-mail: SDT4@Cornell.edu, US;(2) Seminis Vegetable Seeds, 37437 State Highway 16, Woodland, CA 95695, USA, US;(3) Heinz U.S.A., Agricultural Research Department, P.O. Box 57, Stockton, CA 95201-3057, USA, US;(4) Department of Field Crops, The Faculty of Agriculture, Hebrew University of Jerusalem, Kennedy Lee Building, Room 222, Box 12, Rehovot 76–100, Israel, IL;(5) Nippon del Monte Corp., Research and Development, 3748 Shimizu-cho, Numata, Gumma 378, Japan, JP;(6) Nestlé Research and Development Center, Apartado 435, E-06080, Badajoz, Spain, ES;(7) Centre Recherche Nestlé, 101 Avenue Gustave Eiffel, 37390 Notre Dame D’OE, Tours, France, FR |
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| Abstract: | Improved-processing tomato lines were produced by the molecular breeding strategy of advanced backcross QTL (AB-QTL) analysis. These near-isogenic lines (NILs) contained unique introgressions of wild alleles originating from two donor wild species, Lycopersicon hirsutum (LA1777) and L. pimpinellifolium (LA1589). Wild alleles targeted for trait improvement were selected on the basis of previously published replicated QTL data obtained from advanced backcross populations for a battery of important agronomic traits. Twenty three NILs were developed for 15 genomic regions which were predicted to contain 25 quantitative trait factors for the improvement of seven agronomic traits: total yield, red yield, soluble solids, brix×red yield, viscosity, fruit color, and fruit firmness. An evaluation of the agronomic performance of the NILs in five locations worldwide revealed that 22 out of the 25 (88%) quantitative factors showed the phenotypic improvement predicted by QTL analysis of the BC3 populations, as NILs in at least one location. Per-location gains over the elite control ranged from 9% to 59% for brix×red yield; 14% to 33% for fruit color; 17% to 34% for fruit firmness; 6% to 22% for soluble-solids content; 7% to 22% for viscosity; 15% to 48% for red yield, and 20% to 28% for total yield. The inheritance of QTLs, the implementation of the AB-QTL methodology for characterizing unadapted germplasm and the applicability of this method to other crops are discussed. Theor Appl Genet (1998) 97 : 170–180 Received: 27 October 1997 / Accepted: 25 November 1997 |
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| Keywords: | Molecular breeding Germplasm Quantitative traits |
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