Oligomerization of Baculovirus LEF-11 Is Involved in Viral DNA Replication |
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| Authors: | Zhan-Qi Dong Nan Hu Jun Zhang Ting-Ting Chen Ming-Ya Cao Hai-Qing Li Xue-Jiao Lei Peng Chen Cheng Lu Min-Hui Pan |
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| Institution: | 1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.; 2. Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.; 3. Institutes of Life Sciences, Chongqing Medical University, Chongqing, 400716, China.; Ecole des Mines d''Alès, FRANCE, |
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| Abstract: | We have previously reported that baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) late expression factor 11 (lef-11) is associated with viral DNA replication and have demonstrated that it potentially interacts with itself; however, whether LEF-11 forms oligomers and the impact of LEF-11 oligomerization on viral function have not been substantiated. In this study, we first demonstrated that LEF-11 is capable of forming oligomers. Additionally, a series of analyses using BmNPV LEF-11 truncation mutants indicated that two distinct domains control LEF-11 oligomerization (aa 42–61 and aa 72–101). LEF-11 truncation constructs were inserted into a lef-11-knockout BmNPV bacmid, which was used to demonstrate that truncated LEF-11 lacking either oligomerization domain abrogates viral DNA replication. Finally, site-directed mutagenesis was used to determine that the conserved hydrophobic residues Y58&I59 (representing Y58 and I59), I85 and L88&L89 (representing L88 and L89) are required for LEF-11 oligomerization and viral DNA replication. Collectively, these data indicate that BmNPV LEF-11 oligomerization influences viral DNA replication. |
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