Jirovecia branchilis n. sp. (Microsporidia) from glands of Branchiura sowerbyi (Oligochaeta: Tubificidae) in China |
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| Institution: | 1. Hunan Engineering Research Center for Utilization of Characteristics of Aquatic Resources, Colleage of Fisheries, Hunan Agricultural University, Changsha 410128, China;2. Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, College of Life and Environmental Sciences, Hunan University of Arts and Science, Changde 415000, China;3. School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shangdong Province 266109, China;1. Division of Membrane Biology, Biosignal Research Center, Graduate School of Medicine, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan;2. Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan;3. Department of Biological Sciences, Faculty of Science and Technology, Kochi University, 2-5-1 Akebono, Kochi 780-8520, Japan;4. National Institute for Physiological Sciences, 38 Nishigonaka Myodaiji, Okazaki 444-8585, Japan;1. Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China;2. Key Laboratory of Mariculture (OUC), Ministry of Education, Qingdao 266003, China;3. Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;4. Laoshan Laboratory, Qingdao 266237, China;1. Department of Biology, University of Pisa, Italy;2. Centre of Core Facilities “Culture Collections of Microorganisms”, Saint Petersburg State University, Russia;3. Laboratory of Cellular and Molecular Protistology, Zoological Institute of Russian Academy of Sciences, Saint Petersburg, Russia;4. Research Department for Limnology, University of Innsbruck, Mondsee, Austria;5. CIME, Centro Interdipartimentale di Microscopia Elettronica, Università di Pisa, Pisa, Italy;6. CISUP, Centro per l’Integrazione della Strumentazione dell’Università di Pisa, Pisa, Italy;1. University of Cologne, Cologne Biocenter, Institute of Zoology, General Ecology, Zuelpicher Str. 47b, D-50674 Cologne, Germany;2. Technical University of Denmark, National Institute of Aquatic Resources (DTU Aqua), Kemitorvet, Bygning 201, DK 2800 Kgs Lyngby, Denmark;3. School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK |
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| Abstract: | Jirovecia species primarily infect oligochaetes and are typically characterized by large rod-shaped spores with a tail-like posterior prolongation. Presently, seven Jirovecia spp. are reported worldwide with only one described in China. Here, a new species, Jirovecia branchilis n. sp. was discovered in glands of oligochaetes Branchiura sowerybi Beddard, 1892 in China. Jirovecia branchilis n. sp. elicited the formation of numerous opaque xenomas of 0.12 to 0.20 mm (n = 30) in diameter. Electron microscopic observations demonstrated that the earliest developmental stages observed were uninucleate meronts residing directly with the host cytoplasm. Mature spores were rod-shaped with blunt ends and possessed a collar-like anchoring disk, a manubrium-type polar filament, a bipartite polarplast, and a three-layered spore wall. A tail-like prolongation was distinctly observed in the posterior of spores and measured 13.2–28.6 μm long (n = 30). Jirovecia branchilis n. sp. showed 98.54% sequence similarity with Janacekia tainunus isolated from the fat body of chironomidae larvae Kiefferulus tainanus based on obtained partial SSU rDNA gene sequence, but was significantly different in morphology, host, and infection sites. SSU rDNA-based phylogenetic analysis indicated Jirovecia branchilis n. sp. clustered with Janacekia tainanus within the Jirovecia-Bacillidium-Janacekia clade. In conclusion, a new species within Jirovecia, Jirovecia branchilis n. sp. is erected herein based mainly on its morphological, ecological, and to a lesser degree on its molecular characteristics. The whole relationship between Jirovecia spp., Janacekia spp., and Bacillidium spp. is in need of revision and could potentially be elucidated by using additional makers and sequencing a broader diversity of the already described species. |
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| Keywords: | Microsporidia Phylogeny Ultrastructural characteristics |
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