Targeted Gene Delivery to MCF-7 Cells Using Peptide-Conjugated
Polyethylenimine |
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| Authors: | Ahad Mokhtarzadeh Hamideh Parhiz Maryam Hashemi Sara Ayatollahi Khalil Abnous Mohammad Ramezani |
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| Institution: | .Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran ;.Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran ;.Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran |
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| Abstract: | Specific and effective delivery of drugs and genes to cancer cells are
the major issues in successful cancer treatment. Recently, targeted cancer gene
therapy has been emerged as a main technology for the treatment of different types
of cancers. Among various synthetic carriers, polyethylenimine is one of the most
well-known polymers for gene delivery. In this study, we conjugated phage-derived
peptide (DMPGTVLP) to polyethylenimine (10 kDa) via disulfide bonds for targeted gene delivery into breast
adenocarcinoma cells (MCF-7). As negative-control cells, we used non-related
hepatocellular carcinoma cells (HepG2). Peptide-conjugated polyplex exhibited low
cytotoxicity and significantly increased the transfection efficiency in comparison
with unmodified polyethylenimine. Therefore, the peptide-modified vector can be used
as a good targeting agent for gene or drug delivery into breast adenocarcinoma
cells.Electronic supplementary materialThe online version of this article (doi:10.1208/s12249-014-0208-6) contains supplementary material, which is available to authorized
users.KEY WORDS: breast cancer, gene therapy, phage-derived peptide, polyethylenimine |
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