Morphological and molecular analysis of Aggregata aspera n. sp. (Apicomplexa: Aggregatidae) in Amphioctopus ovulum and Amphioctopus marginatus (Mollusca: Cephalopoda) from the Western Pacific Ocean |
| |
| Institution: | 1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China;2. Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China;1. Division of Membrane Biology, Biosignal Research Center, Graduate School of Medicine, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan;2. Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan;3. Department of Biological Sciences, Faculty of Science and Technology, Kochi University, 2-5-1 Akebono, Kochi 780-8520, Japan;4. National Institute for Physiological Sciences, 38 Nishigonaka Myodaiji, Okazaki 444-8585, Japan;1. Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China;2. Key Laboratory of Mariculture (OUC), Ministry of Education, Qingdao 266003, China;3. Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;4. Laoshan Laboratory, Qingdao 266237, China;1. Department of Biology, University of Pisa, Italy;2. Centre of Core Facilities “Culture Collections of Microorganisms”, Saint Petersburg State University, Russia;3. Laboratory of Cellular and Molecular Protistology, Zoological Institute of Russian Academy of Sciences, Saint Petersburg, Russia;4. Research Department for Limnology, University of Innsbruck, Mondsee, Austria;5. CIME, Centro Interdipartimentale di Microscopia Elettronica, Università di Pisa, Pisa, Italy;6. CISUP, Centro per l’Integrazione della Strumentazione dell’Università di Pisa, Pisa, Italy;1. University of Cologne, Cologne Biocenter, Institute of Zoology, General Ecology, Zuelpicher Str. 47b, D-50674 Cologne, Germany;2. Technical University of Denmark, National Institute of Aquatic Resources (DTU Aqua), Kemitorvet, Bygning 201, DK 2800 Kgs Lyngby, Denmark;3. School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK |
| |
| Abstract: | Aggregata Frenzel, 1885 (Apicomplexa) are dangerous protozoan parasites that cause malabsorption syndrome in wild and reared cephalopod species, resulting in significant economic loss to fishery and aquaculture industries. The new parasitic species, Aggregata aspera n. sp., in the digestive tract of Amphioctopus ovulum and Amphioctopus marginatus from an area in the Western Pacific Ocean was identified, it is the second two-host parasite species of Aggregata. Mature oocysts and sporocysts were spherical to ovoid in shape. Sporulated oocysts were 380.6–1,158.4 μm in length and 284.0–1,090.6 μm in width. The mature sporocysts were 16.2–18.3 μm in length and 15.7–17.6 μm in width, with irregular protuberances on the lateral wall of the sporocysts. Sporozoites within mature sporocysts were curled in shape and measured 13.0–17.0 μm in length and 1.6–2.4 μm in width. Each sporocyst contained 12–16 sporozoites. Phylogenetic tree analysis, based on 18S rRNA gene partial sequences, indicated that Ag. aspera forms a monophyletic cluster within the genus Aggregata and has a sister relationship with Ag. sinensis. These findings will provide the theoretical basis for the histopathology and diagnosis of coccidiosis in cephalopods. |
| |
| Keywords: | Octopus 18S rRNA Aggregatidae China |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
