Immunoblotting of mite aeroallergens collected with an indoor Burkard air sampler |
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| Authors: | Masahiro Sakaguchi Sakae Inouye Yuichi Takahashi Susumu Kutagiri Hiroshi Yasueda |
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| Affiliation: | (1) department of Epidemiology, National Institute of Health, Toyama 1-23-1 Shinjuku-ku, 162 Tokyo, Japan;(2) The Yamagata Prefectural Institute of Public Health, Yamagata, Japan;(3) Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital, Sagamihara, Japan |
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| Abstract: | We examined the kinetics of airborne levels of mite allergen particles in a house by combined use of an indoor Burkard air sampler and immunoblotting. Airborne mite allergens collected on the Burkard sampling tape were transferred onto a nitrocellulose membrane, reacted with mouse monoclonal anti-mite allergen (Der pI) antibody, then treated with alkaline phosphatase conjugated anti-mouse IgG. Finally, the blotted allergen on the membrane was reacted with BCIP/NBT phosphatase, and purple spots visible by the naked eye were produced. The shape of the spots was observed under a microscope, and the spot area was measured by an image processor. This technique might be useful for analyzing the behavior of airborne allergen particles in indoor environments. |
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| Keywords: | Airborne allergen Burkard air sampler Immunoblotting Mite allergen |
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