Immunogenicity of hepatitis B surface antigen derived from the baculovirus expression vector system: a mouse potency study |
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Authors: | Roberta Attanasio Robert E Lanford Dennis Dilley Gregory W Stunz Lena Notvall A Burl Henderson Ronald C Kennedy |
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Institution: | Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228-0147. |
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Abstract: | A standard mouse potency test was performed to evaluate the immunogenicity of recombinant hepatitis B surface antigen (HBsAg) produced in the baculovirus/insect cell expression system. Groups of NIH Swiss mice were immunized with serial four-fold amounts of either baculovirus-derived HBsAg adsorbed to aluminum sulfate or a commercially available yeast-derived recombinant HBsAg vaccine preparation. Results from these experiments showed that the effective dose of baculovirus- and yeast-derived HBsAg vaccine preparations necessary to seroconvert 50% of the animals were similar. The duration of the antibody response to HBsAg was studied in mice immunized with the highest doses of the two recombinant vaccine preparations 3 and 6 months after injection. No decrease in the anti-HBs response was observed 6 months after injection. No decrease in the anti-HBs response was observed 6 months after immunization with either of the two vaccine preparations. These results indicate that the baculovirus-derived recombinant HBsAg could serve as an alternative vaccine candidate for hepatitis B virus. |
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