大豆脂肪氧化酶及Kunitz胰蛋白酶抑制剂缺失种质的创新

脂肪氧化酶和胰蛋白酶抑制剂是大豆蛋白中2种重要的抗营养因子。以黄淮海主栽品种鲁豆4号、中品661、豫豆8号、91D15、潍8640作母本，美国引进缺失Kunitz胰蛋白酶抑制剂品种P．I．L83-4387和优良品种Century缺失脂肪氧化酶的近等位基因系Century-2、Century-2．3和Century-1．3作父本进行有性杂交，利用大豆脂肪氧化酶缺失基因及胰蛋白酶抑制剂缺失基因的生化标记对杂种后代进行多年辅助选择，培育脂肪氧化酶缺失基因(lx1、lx2、lx3)、胰蛋白酶抑制剂缺失基因(ti)等优质多基因聚合的大豆新种质，为我国大豆品质育种、生产及加工利用提供优异种质材料。

Development of New Soybean Germplasm with Null Lipoxygenase and Kunitz Trypsin Inhibitor Genes

Soybean is one of the most important sources of plant protein for human.Soybean protein is a kind of high-quality protein composed of balanced amino acids,which contains all kinds of amino acids,especially 8 amino acids necessary for human. But it also contains some components that are not good for human and affect food quality,such as lipoxygenase (Lox) and trypsin inhibitor (Ti).Those are important anti-nutritious factors.Nutritional value and processing quality of soybean can be improved by means of development of new variety with null Lox and Ti.In this paper,New soybean germplasms that pyramided multiple genes of high quality,null lipoxygenase and trypsin inhibitor genes (lx_1,lx_2,lx_3 and ti) were developed by means of cross and biochemical marker-assisted selection of progenies for null lipoxygenase and trypsin inhibitor genes using known Lox and Ti markers (protein markers).Female parents were soybean varieties Ludou 4,Zhongpin 661,Yudou 8,91D15,wei8640 popularized in Huanghuaihai Plain.Male parents were varieties introduced from US,trypsin-inhibitor (Ti)-deficient varieties P.I.L_(83-4387) and near isogenic lines of varieties Century for lipoxygenase (Lox)-deficient genes,Century-2 (lx_2),Century-2.3 (lx_2lx_3) and Century-1.3 (lx_1lx_3).These new germplasms will promote soybean breeding for improved quality production,and utilization.