Production of UDP-N-acetylglucosamine by coupling metabolically engineered bacteria |
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Authors: | Kazuhiko Tabata Satoshi Koizumi Tetsuo Endo Akio Ozaki |
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Institution: | (1) Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6, Asahi-machi, Machida-shi, Tokyo, 194-8533, Japan;(2) Present address: Technical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 1-1 Kyowa-cho;(3) Hofu-shi, Yamaguchi, 747-8522, Japan |
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Abstract: | A production system of UDP-N-acetylglucosamine (UDP-GlcNAc) was established by using recombinant Escherichia coli and Corynebacterium ammoniagenes in combination. E. coli overexpressed the UDP-GlcNAc biosynthetic genes, glmM, glmU, glk, ppa, ack, and pta, whereas C. ammoniagenes contributed to the formation of UTP from orotic acid. Glucose 1,6-diphosphate (Glc-1,6-P2), which was required for the activity of phosphoglucosamine mutase involved in UDP-GlcNAc biosynthesis, was supplied by phosphoglucomutase and phosphofructokinase. Starting with orotic acid (65 mM) and glucosamine (400 mM), UDP-GlcNAc accumulated at 11.4 mM (7.4 g l–1) after 8 h. |
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Keywords: | glucose 1 6-diphosphate metabolic engineering sugar nucleotide UDP-N-acetylglucosamine |
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