Proteomics screening of molecular targets of granulocyte colony stimulating factor in the mouse brain and PC12 cell line |
| |
Authors: | Maryam Ghorbani Amir Hooshang Mohamadpour Soghra Mehri Khalil Abnous Mohammad Hassanzadeh-Khayyat |
| |
Affiliation: | 1. Pharmaceutical Research Center, Department of Clinical Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;2. Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;3. Pharmaceutical Research Center, Department of Medicinal Chemistry, Mashhad University of Medical Sciences, Mashhad, Iran;4. Department of Pharmacology and Toxicology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran |
| |
Abstract: | AimsGranulocyte colony stimulating factor (G-CSF), a new neuroprotective agent, binds to its specific receptors in the brain. In this study we hypothesized that at least a part of G-CSF's neuroprotective effect may be mediated through its interaction with other proteins in the brain.Main methodsUsing an immunoprecipitation (IP) kit, at first the antibody of G-CSF was covalently crosslinked to protein A/G agarose. Then the mouse brain or PC12 cell lysate mixed with G-CSF was added to the agarose beads plus antibody. After immunoaffinity isolation of target proteins, gel electrophoresis was performed and protein bands were identified using MALDI-TOF/TOF and MASCOT software.Key findingsOur data show that G-CSF physically binds to cellular proteins like sodium/potassium-transporting ATPase, beta actin, aldehyde dehydrogenase, regucalcin and glutathione-s-transferase. These proteins are involved in membrane transportation, cell structure, signal transduction, enzymes involve in calcium related cell signaling and redox homeostasis.SignificanceInteraction of G-CSF with these proteins can explain some of its pharmacological effects in the CNS. |
| |
Keywords: | Granulocyte colony stimulating factor Molecular targets Neuroprotection Immunoprecipitation |
本文献已被 ScienceDirect 等数据库收录! |
|