Transgenic mice expressing the <Emphasis Type="Italic">Peripherin-EGFP</Emphasis> genomic reporter display intrinsic peripheral nervous system fluorescence |
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Authors: | Samuel McLenachan Yona Goldshmit Kerry J Fowler Lucille Voullaire Timothy P Holloway Ann M Turnley Panos A Ioannou Joseph P Sarsero |
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Institution: | (1) Cell and Gene Therapy Research Group, Royal Children’s Hospital, Flemington Road, Parkville, VIC, 3052, Australia;(2) Department of Paediatrics, University of Melbourne, Royal Children’s Hospital, Flemington Road, Parkville, VIC, 3052, Australia;(3) Neural Regeneration Laboratory, Centre for Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia;(4) Disease Models Unit, Murdoch Childrens Research Institute, Royal Children’s Hospital, Flemington Road, Parkville, VIC, 3052, Australia |
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Abstract: | The development of homologous recombination methods for the precise modification of bacterial artificial chromosomes has allowed
the introduction of disease causing mutations or fluorescent reporter genes into human loci for functional studies. We have
introduced the EGFP gene into the human PRPH-1 locus to create the Peripherin-EGFP (hPRPH1-G) genomic reporter construct. The hPRPH1-G reporter was used to create transgenic mice with an intrinsically fluorescent peripheral nervous system (PNS). During development,
hPRPH1-G expression was concomitant with the acquisition of neuronal cell fate and growing axons could be observed in whole embryo
mounts. In the adult, sensory neurons were labeled in both the PNS and central nervous system, while motor neurons in the
spinal cord had more limited expression. The fusion protein labeled long neuronal processes, highlighting the peripheral circuitry
of hPRPH1-G transgenic mice to provide a useful resource for a range of neurobiological applications. |
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Keywords: | Peripherin PRPH-1 EGFP Nervous system GET Recombination |
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