| 大熊猫脑钙调素的纯化及性质研究 |
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| 引用本文： | 张燕红,张庭芳.大熊猫脑钙调素的纯化及性质研究[J].生物化学与生物物理进展,1999,26(1):87-89. |
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| 作者姓名： | 张燕红 张庭芳 |
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| 作者单位： | 北京大学生命科学学院,北京,100871 |
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| 摘 要： | 以大熊猫脑为材料，经提取、热处理、Phenyl-Sepharose CL-4B疏水柱和快速液相分子筛层析，分离纯化得到CaM.经SDS-PAGE、 PAGE和IEF鉴定，得到的CaM为一条带.经测定，大熊猫脑CaM的分子质量为19 ku，等电点为3.8.酶活性实验表明大熊猫脑CaM对牛心磷酸二酯酶有激活作用.氨基酸组成分析结果与其他来源CaM相近.
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| 关 键 词： | 钙调素，大熊猫，环核苷酸磷酸二酯酶，疏水层析 |
| 收稿时间： | 1997/10/27 0:00:00 |
| 修稿时间： | : 1997-10- |
Studies on Purification and Some Properties of Calmodulin from Giant Panda Brain |
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| ZHANG Yan-Hong and ZHANG Ting-Fang.Studies on Purification and Some Properties of Calmodulin from Giant Panda Brain[J].Progress In Biochemistry and Biophysics,1999,26(1):87-89. |
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| Authors: | ZHANG Yan-Hong and ZHANG Ting-Fang |
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| Abstract: | Giant panda brain CaM was purified by Phenyl-Sepharose CL-4B hydrophobic chromatography and FPLC. The results of SDS-PAGE, PAGE and IEF indicate that the protein has been purified. The molecular weight and PI of CaM are 19 ku and 3.8, respectively. Giant panda brain CaM shows calcium ion effect in SDS-PAGE and PAGE. It can activate bovine heart PDE. The result of amino acid composition of giant panda brain CaM is similar to CaMs from other sources. |
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| Keywords: | calmodulin giant panda cyclic nucleotide phosphodiesterase hydrophobic chromatography |
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