Hypomethylation of repair patches in HeLa cells |
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| Authors: | T Eremenko F Palitti F Morelli E P Whitehead P Volpe |
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| Institution: | (1) Laboratory of Cell and Molecular Biology, Institute of Experimental Medicine, CNR, Viale Regina Elena 324, 00161 Rome, Italy;(2) Dept. of Biological Chemistry, University of Rome, Rome, Italy;(3) Biology Dpt., DG 12, Commission of the European Communities, Bruxelles, Belgium;(4) Dept. of Organic and Biological Chemistry, University of Naples, Naples, Italy |
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| Abstract: | In HeLa cells, under conditions where normal semiconservative synthesis is suppressed by hydroxyurea, the excision repair process after irradiation by UV results in a small amount of incorporation of nucleotides into nonreplicated DNA. By labelling the cytosine moieties of these repair patches, and measuring the ratio between cytosine and 5-methylcytosine, we have found that the level of methylation of cytosine in repair patches five hours after UV-irradiation of the cells is about half of that observed in normal semiconservatively synthesized DNA. |
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