食线虫真菌28S rDNA PCR扩增片段的RFLPs分析*

本文对16个食线虫真菌(节丛孢属、隔指孢属和单顶孢属)菌株和3个其他相关丝孢菌(顶辐孢属和单端孢属)菌株的28S rDNA 片段进行了扩增, 并用限制性内切酶Sau3A I, Msp I, Rsa I and Hae III对PCR产物进行了消化。采用UPGMA法对 PCR 产物的限制性图谱特征进行了聚类分析。结果表明,捕食性真菌的聚类群与捕食器官类型相对应,顶辐孢属和单端孢属与捕食性真菌的遗传距离较远。该结果与rDNA 的ITS区间、5.8S和18S的序列分析结果一致。

RFLPs ANALYSIS OF AMPLIFIED 28S rDNA FRAGMENTS FROM NEMATODE-TRAPPING FUNGI

Amplified 28S rDNA fragments from 16 isolates of nematode-trapping fungi(Monacrosporium, Dactylella and Arthrobotrys)and 3 isolates of other related fungi(Dactylaria and Trichothecium) were digested with Sau3A I, Msp I, Rsa I and Hae III. The dendrogram was constructed using UPGMA methods based on the distance matrix of combined restriction patterns of the amplified 28S rDNA fragments. The UPGMA analysis results indicated the dendrogram profiles within trapping fungal clades were consistent with types of trapping organs, which supported the results of ITS region, 5.8S and 18S rDNA sequences analysis. Though Dactylaria and Trichothecium generated morphologically similar conidiogenous cells and conidia, they were far from the clade of trapping fungi based on the genetic distance.