Regulation of mouse mammary tumor viral RNA synthesis in embryonal carcinoma cells and in teratocarcinoma derived myoblasts. |
| |
Authors: | M Crépin F Gros |
| |
Affiliation: | Department of Molecular Biology, Pasteur Institute, 28, rue du Docteur Roux, 75015 Paris, France |
| |
Abstract: | The expression of the mouse mammary tumor virus (MMTV) was studied in undifferentiated embryonal carcinoma cells (ECC), in partially differentiated myoblastic cells derived from ECC cells, and in fully differentiated myotubes. Whereas no appreciable amount of MMTV RNA could be detected in embryonal carcinoma cells, hybridation with radioactive viral cDNA revealed relatively large quantities of tumor virus RNA in the teratocarcinoma derived myoblasts. The MMTV RNA level was strongly reduced after differentiation of myoblasts into myotubes. The glucocorticoid hormone dexamethasone which stimulates the MMTV RNA synthesis in differentiated mammary cells did not affect this synthesis in myoblastic cells. By contrast, the apparently repressed synthesis of MMTV RNA in myotubes was almost completely overcomed with dexamethasone. |
| |
Keywords: | MMTV mouse mammary tumor virus ECC embryonal carcinoma cells PCC3 primitive carcinoma cells (clone 3) PCC4 primitive carcinoma cells (clone 4) TNE Tris base NaCl EDTA SDS sodium dodecyl sulfate Dex dexamethasone |
本文献已被 ScienceDirect 等数据库收录! |
|