Micropropagation of lily (Lilium longiflorum) via in vitro stem node and pseudo-bulblet culture |
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Authors: | D T Nhut |
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Institution: | (1) Institute of Biology in Dalat, 116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam, VN |
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Abstract: | A protocol for micropropagation of Lilium longiflorum using pseudo-bulblets from in vitro shoot-tip-derived stem nodes was developed. Shoot tips from stems of dormant bulbs were
cultured on one-half Murashige and Skoog (MS) medium. Stems from plantlets derived from the shoot tips were cut into nodal
segments, which were then cultured on one-half MS medium supplemented with 1 μm 6-benzyladenine (BA). Pseudo-bulblets formed on each node after 1 month. An average of 32 pseudo-bulblets were formed on
all nodes of the plantlet. Pseudo-bulblets gave rise to multiple shoots when cultured on one-half MS medium supplemented with
2.3 μm BA. Shoots were rooted on one-half MS medium containing 1.1 μm
α-naphthaleneacetic acid. A continuous system of propagation by multiplication of pseudo-bulblets with no dormancy period was
developed. The 80 flowering plants produced from shoot tip culture were acclimatized in the greenhouse for 3 months and then
grown in the experimental garden for 8 months.
Received: 29 October 1997 / Revision received: 28 April 1998 / Accepted: 3 May 1998 |
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