Determination of benzimidazole residues in bovine milk by ultra-high performance liquid chromatography-tandem mass spectrometry |
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Authors: | Xia Xi Dong Yichun Luo Pengjie Wang Xia Li Xiaowei Ding Shuangyang Shen Jianzhong |
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Affiliation: | Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. |
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Abstract: | A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC–MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in 8 min was obtained by means of UHPLC. The samples were subject to Oasis MCX solid-phase extraction cartridges for extraction and clean-up. Matrix-matched calibration curves were performed to compensate for the matrix effect and loss in sample preparation. Mean recoveries ranged from 80% to 101% and inter-day precision was lower than 14%. Limit of detection and limit of quantification of the method ranged from 0.01 to 0.5 μg L−1 and from 0.1 to 1.0 μg L−1, respectively. |
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Keywords: | Benzimidazole Ultra-high performance liquid chromatography Tandem mass spectrometry Milk |
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