Structure of a neutral O-specific polysaccharide of the bacterium Providencia alcalifaciens O5. |
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| Authors: | G V Zatonsky O V Bystrova N A Kocharova A S Shashkov Y A Knirel E V Kholodkova E S Stanislavsky |
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| Institution: | Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia. |
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| Abstract: | A neutral polysaccharide containing D-galactose, 2-acetamido-2-deoxy-D-glucose, and 3-acetamido-3,6-dideoxy-D-glucose (Qui3NAc) in the ratios 2:1:1 was obtained by mild acid degradation of lipopolysaccharide of the bacterium Providencia alcalifaciens O5 followed by gel chromatography and ion-exchange chromatography or treatment with anhydrous hydrogen fluoride. On the basis of full acid hydrolysis, methylation, and 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), H-detected heteronuclear 1H,13C single-quantum coherence (HSQC), and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: |
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