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Production and characterisation of monoclonal antibodies to ovine interleukin-5 |
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| Authors: | Bendixsen Tuan Bosward Katrina L Emery David L |
| Institution: | a CSIRO Livestock Industries, Prospect, Sydney, NSW 2148, Australia b Faculty of Veterinary Science, B14, University of Sydney, Sydney, NSW 2006, Australia |
| Abstract: | Monoclonal antibodies (MAbs) were developed against recombinant ovine interleukin-5 (IL-5) produced in the baculovirus expression vector system. One MAb, D11 (isotype IgG1), neutralised the activity of both recombinant and native sources of IL-5 in a biological assay (Baf cell assay) but was only weakly reactive in immunocytochemistry. Conversely, a second MAb, A8 (isotype IgA), successfully detected IL-5 in immunocytochemistry but did not display neutralising activity. The development of these MAbs will enable the assay of ovine IL-5 in vitro and permit studies into the role of hypersensitivity reactions in sheep by neutralisation of IL-5 in vivo. |
| Keywords: | Interleukin-5 Monoclonal antibody Sheep Neutralisation Immunocytochemistry |
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