Synthesis of immunoglobulin by rabbit lymphocytes in vitro in response to anti-immunoglobulin antiserum |
| |
| Authors: | Eileen Skaletsky Stewart Sell |
| |
| Institution: | Department of Pathology, University of California, San Diego Medical School, La Jolla, California 92093 U.S.A. |
| |
| Abstract: | Stimulation of synthesis of immunoglobulin (Ig) in vitro by Con A and anti-Ig in cultures of rabbit lymphoid cells has been analyzed qualitatively using an assay that measures the incorporation of 3H]leucine into newly synthesized proteins, followed by the specific absorption of tritiated immunoglobulin by staphylococcal protein A. Whereas Con A stimulates Ig production by spleen cells only if T lymphocytes are present, anti-immunoglobulin serum enhances Ig synthesis in the absence of T lymphocytes. In contrast, neither Con A nor anti-immunoglobulin serum stimulates peripheral blood lymphocytes to produce enhanced levels of Ig. It is concluded that both Con A and anti-immunoglobulin serum do not activate resting B cells but drive differentiation of B cells which are already synthesizing Ig. Anti-Ig acts directly whereas stimulation of B-cell Ig synthesis by Con A occurs indirectly through stimulation of T cells. |
| |
| Keywords: | Address correspondence to Dr Eileen Skaletsky Dept of Pathology M-012 University of California San Diego La Jolla Calif 92093 |
| 本文献已被 ScienceDirect 等数据库收录！ |
